I have DNA sequences and want to submit in GeneBank ? I have watched some webiner from NCBI , but want more information about the new details they are asking? Thanks
I am looking for displed identification key for ichneumon wasps species to used it for teaching. Thanks
04 May 2019 8,005 2 View
I tried using Fstat but the software gave me Fis and Fst for each of the loci. I am looking for Fst for each of the populations and not loci. Can anyone help me with this or suggestion useful...
10 November 2016 5,098 2 View
I have detected this species in glasshouse whitefly and if anyone have more details of its function in this insects?
10 November 2016 9,647 8 View
I am looking for recommended universal markers to amplify COI gene to using for DNA barcoding studies for a wide range of insect species. Thanks
09 October 2016 4,753 8 View
I have exported data file from GenAlEx but not accepted in STRUCTURE program .
09 October 2016 3,821 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View