We are trying to extract RNA from liver samples that came from animals that were either fed a high fat diet or a low fat diet (Using Qiagen mini kits). Should we use a lipid mini kit for both liver samples, a lipid mini kit for the high fat diet livers and a regular mini kit for the low fat diet livers or does it not really matter?
Dear Isaiah Burciaga
I don't think that using two different kits it would be a good idea. You will introduce variability to the sample preparations and you could find unreliable results. I also use the qiagen lipi tissue kit for RNA extraction from both tissue samples and differentiated cells in vitro and I always find good also with non-fatty samples. I think that the spin columns are optimized to not interfere with lipids but it is not a problem if you start from a non-fatty sample.
All the best.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts