Does the temperature of the optical film need to reach the melting temperature when thermal damage occurs?

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Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,974 1 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

If the weghts of convolution kernel in CNN(convolution neural network) are pre-designable, or weights must be initialized and updated while trainning?

Are the weights of convolution kernel in CNN(convolution neural network) pre-desighable before trainning?

20 June 2024 6,501 6 View

In CNN, is the feature map obtained randomly by convolution kernel?

In CNN(convolution neural network), can the feature map obtained determinately by a random initialization convolution kernel? if not, how to decide the weights in convolution kernel to obtain the...

20 June 2024 6,418 6 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Help me find the full text. I can only find the summary part in many databases, and I can't see the specific content. The author did not reply to me ?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

08 May 2024 9,890 1 View

Help me find the PDF, not only the abstract?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

05 May 2024 8,818 2 View

The components of a pancreatic exocrine organoid using mice pancreas?

I am working on pancreatic cancer. Now, I am trying to generate a pancreatic exocrine organoids using my KPC mice. But after I checking the protocol, PancreaCult™ Pancreatic Organoid Growth Medium...

20 April 2024 3,879 1 View

Any ideas on how to get better bands?

I'm not sure how to interpret or fix the sample so that it creates a normal band. I used Percoll Gradient with density gradients to generate the questionable band. It's supposed to represent...

14 April 2024 9,350 1 View

The pH of the deionized water in the lab is suddenly much higher. Any thoughts why?

One day when we made our regularly used medium, we noticed the initial pH was much higher than usual (above 6.0. usually it is around 4.0). It seems it only happened in our lab. The neighbor lab...

19 February 2024 5,250 4 View

Using OBD technique i am trying to measure laser induced shockwaves velocity i found that at start velocity increases and then decay?

i am unable to interpret why its increases in start as shown in figure

11 August 2024 2,175 1 View

Where can I find the recent population of Indian cities (2020s) ?

Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!

10 August 2024 856 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,684 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,986 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,289 1 View

Can anyone explain why there is not any color bands in the middle portion of the tensile coupon?

Details of the Analysis. Static Analysis Composite Layup Continuum Shell Elements FRP Material (Elastic and Hashin Damage)

03 August 2024 8,537 4 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,465 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,121 4 View