If any molecule such as small peptide or carbohydrate gets conjugated with graphene oxide, will it affect the inter-layer distance, i.,e, whether the incorporation will be in between the layers?
Intercalation of molecules in the interlayer of graphene oxide have been intensely studied. The interlayer of graphene oxide is increase with Intercalation of groups. Increasing of interlayer spacing could confirmed by AFM and XRD techniques. The layer distance between graphene oxide based on XRD patterns can be calculated by the Bragg's equation λ=2dsin(Ɵ):
where λ is the wavelength of the X-ray beam, d is the layer distance or interlayer spacing and Ɵ is the diffraction angle obtained from XRD pattern.
If all these values are substituted in Bragg's equation ,interlayer spacing or d-spacing can be calculated
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