I´ve fixed diminazene stock solutions 200 ug/mL on DMSO, sterilized it by filtration and kept on refrigeration (4°C), at the beginning the solutions looked as a translucent one, but several days before (7-10) it became cloudy...
I am looking for expert recommendations on qualitative analysis software. I have been engaging in more mixed-method work recently and I need reliable qualitative analysis software. Any...
02 February 2021 7,984 7 View
I would like to perform comparisons between densitographic profiles from 4 different experimental conditions (1 control + 3 treatments), but each one comes from 20 experimental replicates. I...
29 March 2020 5,531 3 View
In every simulation I've ran, the cutting forces spike up and down instead of staying at a fairly constant value. Why is this happening? I've defined a rigid body reference point on the cutting...
23 January 2020 9,059 3 View
I am interested in any published data on how percentage of different age stages (juveniles, subadults ...) change depending on the type of the taphocoenoses etc?
05 December 2018 7,094 4 View
I know most people recommend R over SPSS and SAS--particularly for multi-level modeling--but I have yet to read WHY R is superior for these kind of analyses. I am preparing to run some HGLM and...
03 August 2018 2,104 7 View
Does anyone have an exhaustive list of the HNF1b target genes? Are GLUT2 and INS few of them? thanks!
12 January 2017 4,446 2 View
i have some troubleshooting about protein agregation in western blot, because i saw bands in higher molecular weigth, but they are not innespecific, because i had negative controls, is the case...
17 November 2016 9,004 2 View
There are objects that have different shapes but nevertheless have the same topological dimension. How can you get the topological dimension of an object?
01 January 1970 8,120 5 View
i would to know some of the research gaps in the artificial intelligence field in most african countries.
03 March 2021 6,145 3 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...
28 February 2021 1,556 6 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View