Is it the right method to transform path coefficients into correlations before meta-analysis?

We all know it is possible to get path coefficients from correlation matrix (e.g., PATH ANALYSIS I: INTRODUCTION (ecu.edu) ). Using a simple solver on a spreadsheet, it is therefore possible to...

23 February 2021 8,366 3 View

How can one use PSO to find finite non continous parameters (e.g. enums) ?

Hi, I am making an implementation of PSO for deep neural networks training. For that, I would like the PSO to find the best activation functions as well. However, unlike weights, I use a set of...

12 November 2020 1,506 3 View

Interactions in non-experimental data (agriculture) ?

24 September 2020 2,954 5 View

How to identify trees that have not recovered but are not yet dead after transplanting in urban environnement ?

Are there reliable and easy-to-measure criteria to identify trees that have not recovered after transplanting, but are not yet dead? In particular, I seek to know the reliability of criteria such...

31 August 2020 728 2 View

Any advice for an econometric model with panel data and a lagged variable ?

Hello, I am studying the nutrition status of households using a panel data survey of South-African Households. I would like to study the changes in the nutritional status of households between...

23 April 2020 5,017 6 View

How can I get the required sample sizes for PLS-SEM using G*Power?

I have tried to compute sample size with G*Power but I cannot get the results indicated in the table 1.7 of the book by Hair et al. A Primer on PLS-SEM. Have you tried to check this ? Have you get...

24 March 2019 5,596 1 View

Who knows a botanist in Mexico who could help me by collecting Mexican species of Gentiana?

Because of time limitation, I will not be able to visit Mexico in summer 2019. Yet, I would need a few species from there for some phylogenetic work by autumn 2019. These species are: G....

02 March 2019 9,199 2 View

Rate of oxidation of various materials?

Dear colleagues, Would any of you guide me to literature papers or websites which compiled the temperature dependent (T>1000K) oxidation rates of various materials (metals, ceramiques,...

23 January 2019 1,746 9 View

Does anybody have experience in the in vitro generation of IFNy-secreting NK-cells from murine progenitors (LSK) ?

28 May 2018 7,911 3 View

What is the simplest and cheapest method for coating glass with a thin 100 micron uniform layer of epoxy or silicone resin?

I want to make a uniform thin coat of resin /pigment on 5*100 cm glass sheets. The glass could be cut before or after the coating is applied. The coating should be very even in thickness. Any...

10 February 2018 2,569 5 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

How to analyze dendritic spines from tissue (Prelimbic cortex)?

Hi there, I am trying to measure dendritic spines from mouse fixed tissue, specifically, I am looking at the prefrontal cortex. I injected the PrL with a lentivirus to knock-down my protein of...

27 January 2021 9,251 2 View