How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is it possible to unsort cases in spss after data has been sorted by Split file (Sorting Data)?

Hi Everyone, i sorted the data based on some demographic variables by "split file" option for the comparison of groups ( now i donot remember the order of selecting the...

07 October 2020 7,571 3 View

Why don't my isolated mouse cardiomyocytes contract?

I'm trying to isolate left ventricular myocytes from wildtype C57BL/6 mice. Currently the isolation step seems to be going okay - usually I get >50% viability of cells (although this is...

16 September 2020 4,335 3 View

How can i measure the serum concentration of crude plant extract administered to experimental rats?

I'm conducting a toxicological experiment. I administered crude ethanol extract to pregnant rats. I need to check two things -the serum concentration of the extract in the dam rats...

11 August 2020 1,914 2 View

Merge files in spss, with one big and one small database?

I am trying to merge 2 datafiles which both contain valuable information in SPSS. The big one has 4000 persons in it, and a couple of variables. amongst them, 40 places of birth. I want to add the...

11 May 2020 4,608 1 View

Do children have an obligation to look after their parents?

It is a common belief that children must look after their parents due to the fact that the parents had sacrificed so much for their children. In terms of educating, feeding, caring and giving...

27 March 2020 9,742 9 View

During a muscle contraction by lower to medium motor unit groups, how do high threshold muscle fibers shorten? Passive? Active?

During a muscle contraction by lower to medium motor unit groups, how do high threshold muscle fibers shorten? Passive? Active? So if I bend my arm in the elbow without weight in my hand, how do...

04 March 2020 3,313 1 View

What is the best gift that you can give to your children?

Parents give birth to children. Parents would be very much fond of children, playing with them, seeing in them the happiness and excited and attracted by their innocence and their behaviour. As...

17 February 2020 5,300 96 View

Which Statistical Test to Use?

01 February 2020 4,751 3 View

What is the best gift that you can give to your children?

Parents give birth to children. Parents would be very much fond of children, playing with them, seeing in them the happiness and excited and attracted by their innocence and their behaviour. As...

01 February 2020 2,529 7 View

What material should be preferred for manufacturing best quality heat shield?

Object while returning into atmosphere attends Hypersonic speed and to save what's inside, what material should be used to manufacture heat shield.

01 February 2020 8,649 2 View