Does cold plasma have effect on pectin modification?

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How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What happen if a transcription factor bind directly to not only the regulatory region but also the target gene sequence?

As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...

13 May 2024 1,099 3 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

Can I use a different grade of cells for my positive control?

I am performing PCR as a QC test to look for a transcription gene that should be negative after a CAR T therapy process. As we are comparing against a CAR transduced patient's cell, we require a...

22 April 2024 5,262 1 View

How do you remove snow particles on cryoboxes that were stored in LN2?

I have difficulty on removing snow particles on cryoboxes, storing cryovials. The snow particles can sometimes take a lot of space and causing difficulty in closing the cryobox which will later be...

19 February 2024 6,690 0 View

Can I merge T cell from two different host?

My lab co-worker need a lot of T cell for his animal experiment, however one of the donor can't provide enough for downstream analyses, we thought to combine purified T cells from two donors in...

06 November 2023 7,188 0 View

Does the computer system architecture of RFID toll system in Malaysia uses pipelining? Where can I find information about it?

I was told to propose a computer system architecture design to overcome the problem of RFID toll system and compare the proposed design and existing design about the implementation of pipeline.

25 July 2023 2,961 0 View

Are there some new-discovered exposures in the context of gestational diabetes mellitus?

Especially related to nutritions.

14 July 2023 1,072 3 View

Cytoscape loading node error?

I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...

03 July 2023 9,412 0 View

What cause the Gloo after incubation using Qiagen DNA extraction kit?

I was performing the DNA extraction of cells with a concentration of 5x10^6. After adding AL buffer and proteinase K an incubation was done. After the incubation period at 56 degree Celsius, I...

27 June 2023 9,761 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

06 August 2024 8,716 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Appearance of crystallization peak in heating cycle of DSC test?

For which types of polymers, the crystallization peak in heating cycle of DSC test can be observed and for which types cant? In another word, when this exothermic crystallization can be observed...

18 July 2024 348 3 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Has anyone simulated Argon plasma jet in Comsol?

Hello, Has anyone successfully modeled 2D argon plasma jet using the COMSOL? I would greatly appreciate any guidance on this matter.

30 June 2024 5,597 0 View