What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution?

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution? When I am doing the experiment, I find it is difficult to accurately determine the total...

04 January 2021 2,694 1 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

Should I use the Nafion 211 as proton exchange membrance in microbial fuel cell?

I'm currently using Nafion 211 for the proton exchange membrane in my microbial fuel cell experiment. I found lots of articles recommended to use Nafion 117 instead. My experiment was failed,...

11 November 2019 9,326 3 View

How to calculate effect size in R with Metafor package?

07 October 2019 8,027 3 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

03 October 2019 9,077 2 View

Where i can download data of protein/gene interaction networks, preferably in matlab format?

Dear friends, i am a researcher on numerical computation, especially interested on PageRank computation. Now, I am teaching in a agriculture university, thus i want to do some applications to...

27 August 2019 7,598 4 View

How to stain T cells for Ki67/Granzyme B and other surface activation markers together?

Could anyone help me with staining T cells for surface activation markers (CD69) and Ki67/Granzyme B at the same time? The Ki67 staining protocol requires 70% ethanol treatment which might be too...

13 August 2019 7,513 4 View

What is the optimal diameter of the beads for extracting fungal DNA?

I want to extract fungi and bacteria DNA from skin swab. Some articles used two 5mm steel beads per sample, some used 0.4-0.6mm ceramic beads per 0.5g sample. And other diameter for beads were...

05 August 2019 6,471 1 View

How do cancer cell migraton and invation?

Recently, I want to use transwell assay to study glioma cells migration and invation. I have looked for a lot of protocols. And there were different opinions. I didn't find a complete and...

07 July 2019 1,175 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View