My objective is to evaluate pharmacological activity of source plant (capsicum fruits) extract. I have received heterogeneous suggestion pointing that high amount of organic solvents could reduce some antioxidant and phytonutrients properties. Moreover, during organic extraction high temperature might degrade the protein/enzymatic activities. However, my priority is not to emphasize on isolation/purification of bioactive compound.
Hence, does aqueous extraction is better than organic extract for evaluating pharmacological activity of capsicum (fruits) extract?
In the case that you intend to study the biological activity in the essential oil of the pepper extracted by hydrodestilation, the essential oil and the hydrolate have a greater activity than the extracts extracted with organic solvents such as hexane.
@ Abbas Choudhury
In aqueous extract, solvent is water. But all the phytocomounds may OR may not get extracted in case of water. One has to compare the outcome of the phytocompounds obtained by the use of water, polar and non polar solvents. Any solvent which will yield more number of phytocompounds is the best one.
n that case, I recommend that you make use of a phytochemical study using column chromatography, where secondary metabolites are extracted using different solvents in increasing order of polarity and thus have the different fractions.
It depends on the solubility of bioactive constituents of your plant extract. Solvent types affect the biological activities of the extracts. Solvents differ in the extraction capabilities depending on their polarity and on the solute’s chemical structure. Different solvent extracts have different soluble phytoconstituents in different amounts and hence, they have varying degrees of biological activities. Therefore, you can compare the biological activity (the exact pharmacological property you wanna test) of the aqueous and different organic extracts and choose the higher one.
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Some studies have shown that methanol extracts more phytochemicals compared with other solvents. Some other studies have favoured hydromethanol. Personally, I feel the properties of the bioactive compound you are interested in should drive your choice of solvent.
Article Effect of extraction solvent on total phenol content, total ...
Check this out.
The answer must depend on the active constituent which be separated ...??
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