Does anyone know which packaging plasmid I should use with retroviral transfer vector PIG pMSCV?

More Ximena Garate's questions See All

Oxygen activation by platinum?

Good morning. Does anyone know how oxygen activation occurs on CeO2 catalysts by platinum addition? I have read articles that mention that this occurs, however they do not explain how or why it...

26 January 2022 5,976 2 View

Matrigel (900794) stock concentration?

Hello. I received a Matrigel from Collaborative Biomedical Products with catalog number 40234 and lot 900794. I have already search on the corning website but there is no information about this...

16 June 2021 2,657 0 View

Is there someone else working on the dynamics and ecology of coarse woody debris (CWD), snags and leaf litter in tropical forests?

We have worked on this topic in three types of forests in the ecuadorian Amazon along altitudinal gradients and we want to link these data and projects to global and regional networks of tropical...

09 August 2020 3,052 5 View

Does someone have experience using a Teledyne Photometrics camera? Are those good cameras?

I am looking for a PRIME BSI, a back-illuminated sCMOS camera, to be used in a Spinning Disk Confocal Microscope. This camera is much cheaper than an Andor camera. I would say that from the...

28 November 2019 8,853 0 View

Has anyone ever Tirfing fixed cells mounting in Fluoromount G media?

I am not sure if Fluoromount G is a good media to perform TIRF because his refractive index. Has anyone tried?

24 January 2019 1,916 2 View

Which longitudinal analyses should I choose?

I am trying to decide which longitudinal analysis I should choose for my study. I collected data at four different time points: 1. Before the intervention 2. After the intervention 3. Three...

15 November 2018 5,123 5 View

NFATc1 Westernblot Bands

Hello! I am using an anti-NFATc1 Santa Cruz antibody (7A6, sc-7294). The company predicts the molecular weight for the isoforms in 90; 110 and 140 kDa. My problem is that I hardly get to see these...

25 June 2017 5,567 4 View

7H9 becomes cloudy but nothing is growing in the sterility plates after 10 days of incubation, what coul it be?

I set up 10 subcultures of MTBC from frozen aliquotes. Two grew well and 8 became cloudy after 7 days in the incubator. I thought it was bacterial contamination so, I repeated the subcultures. The...

06 April 2016 8,255 7 View

What RNA isolation kit or extraction protocol would you recommend for soil samples? What protocol would you follow to separate 16s and 18s RNA?

I'm studying soil microbial communities response to nutrient addition and cattle grazing (cows) in the Pampean Region, Argentina. I'm doing DNA amplicon sequence analysis for bacterial and fungal...

14 February 2016 6,122 7 View

Who can identify this pollen grain?

Pollen from surface soil sample, taken in Namibia in the Tsumeb region. P=15um

10 December 2015 6,677 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View