I am working on collecting DNA from B cells from human lung samples. We have been working with Invivoscribe to have these go through NGS but have been unsuccessful thus far. Where else can we ship samples to for sequencing?
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
One of the challenges in ensuring quality and integrity in blended learning modules is cheating especially when it comes to assessment (Tests, exams, and assignments). Now one of the solutions...
21 July 2024 6,741 0 View
Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...
19 July 2024 8,389 9 View
Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...
16 July 2024 7,205 2 View
Hi all, if you are looking for PhD and Master opportunity to work on Microalgae research and Biosensor for bacteria detection. Please do not hesitate to email me [email protected]
07 July 2024 7,576 0 View
The photo-electric effect does not support quanta in light, since a light particle moving towards a surface cannot cause an electron to move away from the same surface at a right angle. Instead, a...
06 July 2024 2,288 35 View
Newton found the law of gravity - but did not understand it. Fatio understood the law - but he was not accepted. Fatio assumed fast particles moving in all directions. Stability in planetary...
30 June 2024 9,284 28 View
Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...
27 June 2024 5,068 1 View
In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...
15 June 2024 3,884 1 View
I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...
10 June 2024 5,941 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids
28 July 2024 4,985 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?
27 July 2024 5,916 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View