John Sullivan

5 Questions 2 Answers 0 Followers

Questions related from John Sullivan

Has anyone used AAV to successfully express high levels of a protein of interest in heart muscle cells in vivo?

I am seeking to use in vivo injection of AAV in adult mice to express TEV protease in to the heart muscle cells of our mouse line. We do not have experience with this, but based on the literature,...

26 April 2021 9,297 1 View

What are the best activation markers for T cells in human lungs?

Our lab is looking to buy antibodies that are markers for activated T cells in human lung tissue. Has anyone tried something like this before? What did you use?

04 February 2020 440 2 View

Does anyone know which companies reliably have assays for Next generation sequencing of B lymphocytes?

I am working on collecting DNA from B cells from human lung samples. We have been working with Invivoscribe to have these go through NGS but have been unsuccessful thus far. Where else can we ship...

13 January 2020 2,216 0 View

How does Laser Capture Microdiessection affect integrity of DNA?

I am capturing B cells from Lung tissue using LCM, and then isolating the DNA for various downstream assays. When I finish DNA isolation using the Qiamp mini kit, I am getting good quantity and...

24 September 2019 4,790 3 View

How do I avoid destroying my surrounding tissue when collecting cells with IR laser in Laser Capture Microdissection?

I have been staining murine lung tissue with Immunofluorescence to collect B cells using Laser Capture Microdissection. The staining looks great, but when I would test the IR spots to collect...

19 July 2019 1,066 1 View