I would like to irradiate my cells with 40J/m2 of UV C, but the UV crosslinker at our facility has the UV A lamp. I would like to know if it is possible for irradiating my cells with equivalent UV A instead of 40J/m2 UV C.
The case is not as simple as you are thinking. The damage due to UV irradiation is not only due to the dose or intensity you are providing. The wavelength of the light plays the major role. UVC means it is around 254 nm which could get absorbed by almost all the biomolecule and undergo harmful photochemical reactions. UVA is around 365 nm which is much longer compare to UVC, probably not suitable for desired photochemical reactions on there, even though the irradiation is for a longer duration.
The case is not as simple as you are thinking. The damage due to UV irradiation is not only due to the dose or intensity you are providing. The wavelength of the light plays the major role. UVC means it is around 254 nm which could get absorbed by almost all the biomolecule and undergo harmful photochemical reactions. UVA is around 365 nm which is much longer compare to UVC, probably not suitable for desired photochemical reactions on there, even though the irradiation is for a longer duration.
as suggested by Pal.
I think its not possible to use UVA instead of UVC.
regards
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