In our lab we have tons of tissue samples stored in RNA Later and we're about to start the RNA extraction for PCR. I'm in search of the best fit protocol. We don't have a tissue homogeneizer, we would accept suggestions for that too. Extraction kits are not an option either, we need to run it in the cheapest way possible.
João Pedro Simões
You have so many limitations and thus getting the "best" is not the suitable Word you used.
Nevertheless, you can use either manual grinding (always with liquid nitrogen) or with the help sterilized and RNAse free glass beads to homogenize the tissue samples and use phenol:chloroform method OR trizol method for the extraction of RNA.
But since you lab is not equiped with enough required possibilities, I would recommend you to plan and look for a good and complete strategy for your experiment before actually starting the experiment. Also, perform a mock test to see if your strategy will work, Before starting with real samples.
Senthil Kumaran I guess he was asking for the RNA extraction protocol and not the storing. You can suggest the extraction protocol if you have one.
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