João Pedro Simões

3 Questions 3 Answers 0 Followers

Questions related from João Pedro Simões

Which and for how much time kan I keep Western Blotting solutions?

I'm starting a series of WB experiments in my lab, and it'll be the first time that I do this technique. I read a bunch of protocols and realized that is required significative number of different...

10 October 2019 1,614 4 View

Does anybody have a protocol of ihc of paraffined samples for ki67?

We develeped a PDX model of osteosarcoma, using fresh tumor samples. Now we need a comparison of protein expression in the original tumors and the mouse tumors. I have never done ihc and was...

03 March 2019 3,667 3 View

Does anyone know the best way of RNA extraction for osteosarcoma tissue samples?

In our lab we have tons of tissue samples stored in RNA Later and we're about to start the RNA extraction for PCR. I'm in search of the best fit protocol. We don't have a tissue homogeneizer, we...

03 March 2019 5,333 2 View