How do I estimate the real value of G2 cells on ModFit?

I've performed some cell cycle flow cytometry experiments and the software always shows a default value for Dip G2 cells (8%). What should I do with these 8%?

10 November 2014 1,079 4 View

How do you interpret the experimental data of cell cycle by flow cytometry?

I've been using BD's "cell cycle test plus" kit and I am a beginner in this kind of experiment. The values are shown in percentage of diploid and tetraploid or aneuploid cells on G1, S and G2/M....

09 October 2014 4,507 12 View

What should I do with tetraploid and aneuploid cells on the results of flow cytometry?

09 October 2014 2,303 5 View

Down-regulation of Bcl-2 and up-regulation of Bax and CIDEA. Does this ALWAYS mean apoptosis?

09 October 2014 8,678 8 View

Is LDH assay able to evaluate possible mechanism of action related to necrosis?

08 September 2014 1,729 10 View

Is it necessary to "synchronize" the cell cycle before drug treatments? And what drug concentration is recommended to use in cycle test analysis?

08 September 2014 1,520 5 View

What is the "general consensus" on flow cytometry about cells with FITC-annexin+/PI+?

08 September 2014 759 18 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View