8 Questions 24 Answers 0 Followers
Questions related from Luis Felipe Buso Bortolotto
I've performed some cell cycle flow cytometry experiments and the software always shows a default value for Dip G2 cells (8%). What should I do with these 8%?
11 November 2014 1,147 4 View
I mean some free and/or online reliable tools.
10 October 2014 4,669 6 View
On my work, flow cytometry experiments with treated tumor cells point to cell cycle arrest on G1 and apoptotic death, but there's no p53 and caspase expression. PCR array experiments were done...
10 October 2014 8,728 8 View
"Cell cycle flow cytometry" question (again): Since results are discriminated in diploids and tetra or aneuploids, how should I interpret these values? I explain: most of the cells are determined...
10 October 2014 2,360 5 View
I've been using BD's "cell cycle test plus" kit and I am a beginner in this kind of experiment. The values are shown in percentage of diploid and tetraploid or aneuploid cells on G1, S and G2/M....
10 October 2014 4,582 12 View
Some think that cells that are positive for both dyes (PI and FITC) are in late apoptosis, while others say they're on late apoptosis/early necrosis or just necrosis.
09 September 2014 855 18 View
Since LDH assay determines membrane integrity, may this test be sufficient to conclude that there is a necrosis cell death-related induced by drugs?
09 September 2014 1,787 10 View
This question is for those who have already used the BD Cycletestet Plus: I'm using this kit for cell cycle arrest analysis on cancer cells subjected to treatment with natural compounds. Some...
09 September 2014 1,595 5 View