Does anyone know PCR primers for the detection of mammalian DNA in ticks?

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Does anyone know PCR primers for the detection of mammalian DNA in ticks?

I want to look for galactose-alpha-1,3-galactose in ticks and for the presence of mammalian blood at the same time. I only found primers for vertebrates or species specific ones. But i only need...

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Alisa Frank

thank you, but i wanted to avoid sequencing.

Elmara Graser

May be you can amplify the Myostatin gene. This gene have only mammals and birds

Hossein Khodadadi

If you searching for presence of human blood in the ticks, you may use beta globin primers. you can find or perches them easily.