Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

What is the most adequate (serum) dilution to use to determine IgE in mice serum via ELISA assay (commercial kit)?

We have a commercial kit from RayBioTech to determine IgE from mice serum. In accordance with the supplier's instructions, we have to use a dilution of 1:10000 of the serum. Is this dilution the...

06 January 2021 4,217 3 View

Is there any reference levels of IgE in healthy mice serum, like there are for other biochemical processes/components?

My question goes for reference levels of IgE in mice serum. I know that there are tables for the most common biochemical processes/compounds (red blood cells, white blood cells, AST, ALT, etc),...

06 January 2021 8,215 3 View

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It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

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13 December 2020 3,861 3 View

How can I do the bang's blinding index and james blinding index?

Hi! I would like to know with which statistical software the bang blinding index and the James blinding index and how to do it. Thanks

09 December 2020 1,363 2 View

Do anyone know a lab in Mexico where dielectric properties of solids and liquid can be measured?

I mean those kits that can be connected to vector network analyzers in order to find out permittivity and permeabilitty of solid and liquid from around 1 to 10 or 30 GHz

25 November 2020 852 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell.?

Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell. And he talked about one of them?

09 February 2021 1,099 8 View

Science Teacher's Attitudes Towards Career Learning Index among Senior School Students in Chemistry?

Full discussion of this project topic

09 February 2021 2,534 3 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Hi, Good day! Do you have related studies about my title, "Factors Affecting the Senior High School Students' Stress Tolerance in Problem Solving"?

I am new researcher. I want to learn more and I need your help. Thank you.

04 February 2021 6,834 4 View