We would like to mark nocturnal animals with something that we can see with an infrared camera, and ideally use in a way to be able to differentiate between animals. Does anyone have any experience or suggestions of something we can use?
Thank you, Aparna Sathya Murthy !!! That is very helpful. Are those considered safe to use on animals? IACUC-approved?
Thank you!!
The species we are developing an qPCR assay for are the below: Ascaris lumbricoides (roundworm), Ancylostoma duodenale (hookworm), Necator americanus (hookworm), and Trichuris trichiura...
08 September 2015 7,626 2 View
We are going to test deer mouse fecal samples for glucocorticoid levels. However, we are unsure if grinding feces in methanol is adequate for viral inactivation. Any guidance/input would be great.
09 October 2014 6,709 2 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...
27 February 2021 4,218 7 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...
22 February 2021 6,386 1 View
Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...
22 February 2021 3,322 3 View
The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...
20 February 2021 9,226 2 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View