Does anyone know how to genotype of MHC allele (g7/g7, g7/b, b/b) please, e.g., using microsattelite?

Guangzhi Zhang @Guangzhi-Zhang-6

09 June 2016 0 7K Report

Does anyone know how to genotype of MHC allele (g7/g7, g7/b, b/b) please? e.g., using microsattelite. Thank you.

Badges
Science topic

More Guangzhi Zhang's questions See All

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Articles on" Gender disparities i leatherwork education"?

Articles on" Gender disparities i leatherwork education"

07 August 2024 2,500 0 View

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Genotype and allele distribution statistics ?

Hello, how can I do the statistics associated with genotypes and alleles as in the table? thank you very much for your attention.

17 July 2024 7,606 2 View

Chi-square test for allele distribution?

Hello, when calculating the p value for the alleles in the table, how do we place the values in the chi-square test in the four-eyed table? Thank you very much for your attention.

17 July 2024 998 2 View

Why band for normal allele appears in a sample that is confirmed homozygous mutant?

I use confirmed homozygous mutant and homozygous normal samples for tetraARMS PCR optimization. everytime I run amplified products on gel I observe three bands instead of two bands. DNA samples...

27 June 2024 8,403 3 View

Population coverage analysis for some MHC II alleles?

Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...

20 June 2024 2,155 1 View

How can i select positive homozygous and heterozygous controls for SNP genotyping?

Two genes are PNPLA3 and TM6SF2

01 June 2024 8,047 1 View

Uncallable SNP showed significant to MTA, what to do?

I am getting a significant marker trait association for traits. But understanding the allelic association is difficult; NN is the missing allele. Can anyone help me?

13 May 2024 8,860 2 View

How to convert SSR allele scoring (bp) to rqtl/Joinmap format for linkage & parentage analysis (A, H, B, etc) ?

Hello, I am planning to conduct linkage and parentage analysis using microsatellite markers (SSR). The scoring process has been completed with output in allele size (bp) format. The analysis is...

12 May 2024 5,329 0 View

How do I choose a suitable connection network for sPCA?

I am currently running a sPCA on GBS data from a number of plant populations following the tutorial provided by Thibaut Jombart. I understand one important decision to make during the analysis is...

04 April 2024 7,959 0 View