I want to measure the total carotenoid content of watercress leaves. I'm using 25μg powder of freeze-dried watercress and 10ml of acetone for the extraction process. I'm measuring the absorbance in a UV-Vis Spectrometer. Which are the best equations to use for the correct quantification of total carotenoids?What about the Wellburn and Lichtenthaler ones? Information in the literature is a mess. It seems that everybody is using whatever they think is correct.
I think that the Lichtenthaler method (paper below) is quite appropriate. At page 366 you can find the equations for either 80 or 100% acetone as pigment solvent.
H. K. LICHTENTHALER, 1987. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. METHODS IN ENZYMOLOGY, VOL. 148, pp 350-382.
@George Zervoudakis Could you plz send the paper you mentioned. i tried to download but it requires purchase....
H. K. LICHTENTHALER, 1987. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. METHODS IN ENZYMOLOGY, VOL. 148, pp 350-382.
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