Hi,

Could you elaborate on what type of analysis you'd like to do with mass spectrometry? Do you want to analyze a protein, or a mixture of proteins? Or small molecules? This will determine the type of sample preparation you'll need to do. And are you looking to perform an electrospray experiment, or MALDI?

In general, your liquid samples should be acidic (to aid analyte ionization) and devoid of non-volatile constituents as these will clog the MS.

Hope this helps, looking forward to some more information from your side.

You are right Eef.   Hasan needs to give more details. Because MS can be use with different instruments.   Liquid samples can directly infused to MS for parent and product ions selection to develop the analytical methods.  

There are so many pre treatments are available but before that the question that you ask that will requires further details.

Hi all

thanks for your answer. I need to analysis stable isotopes by using mass spectrometer from insect. 

Hi,

Given the limited information you provided, I guess upon solubilization of the proteins from insect (cells) as described above, you will need to mix some samples (stable isotope-labeled and non-labeled) and proteolytically digest these into peptides. Consequently, the resulting peptide mixture can be analyzed by reversed phase LC-MS/MS, enabling both qualitative identification of proteins, as well as quantitation of their relative abundances using the stable isotope-labeled peptides.

Hope this helps!