I have tried putting negative electrode at the right arm and positive at xiphoid area for D2 and negative at right leg and positive at left arm for AVF, but I get indeterminate axis in hypertensive rats
Thanks
Why did you put electrodes on xyphoid area? Do you need 3D axis?
If you need only 2D then: http://en.wikipedia.org/wiki/Einthoven's_triangle
If you need 3D, then you can place a few electrodes on the chest to get the "frontal plane" data.
Using the Einthoven triangle simply pick the two longest vectors to determine axis. Polarity is not a real issue, if you swap exploring and reference electrodes, you will see the mirror vector pointing to opposite direction but amplitude is correct.
I obtain electrocardiograms (ECGs) using a Nihon Kohden ECG-6851 single-channel electrocardiograph (Nihon Kohden, Tokyo, Japan), with electrodes that were modified to maximize contact with the shaven skin of the rat (elbows and knees (shaved and alcohol 70)) . Registers were taken in the three bipolar derivations (I, II and III) and in the unipolar limb derivations (aVR, aVL and aVF). Paper speed is
50 mm/s and sensitivity is 1 mV/cm.
I calculate the heart axis using two methods. Both of them are commonly used:
a) Tilley´s table in I and III derivations
b) Mathematical formule: The formula used in this website http://www.my-ekg.com/formulas-calculos-ekg.html is based on the calculation of the frontal QRS axis using the I and AVF derivations and trigonometric calculation by magnitude of the vectors.
If you need something more only you need contact with me by mail.
Mucha suerte !!!
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