Hi, recently the lab lost all the JM 109 competent cells of the Pgem T easy vector kit, so we found E. coli JM 1452 cells, Anyone knows something about this strain?
We are seeking to count cell death stain from confocal image stacks; however, Neurolucida is quit expensive. Are there any alternatives that you recommend? Is ImageJ viable?
08 October 2023 1,263 1 View
Hello, I am trying to double-stain with Pax 6 and Cleaved Caspase 3. I have looked into the markers but unfortunately, both antibodies are produced in rabbits. I tried Pax 6 and Conjugated Cleaved...
27 November 2022 3,145 2 View
At the laboratory they are using a glycohemoglobin reagent set (Teco diagnostics), but I do not know how to "translate" those results into HbA1c values, so I can classify people into healthy...
06 March 2018 1,793 4 View
Dear colleagues. I'm trying to perform a WB to validate antibodies and I'm using Human ApoB100 (>95% SDS-PAGE) as a standard in the gel. I have tried two different loadings of protein 1.5 and 4...
31 December 2016 2,447 2 View
Dear researchers, I was reading about drinking water and fecal contamination. I found different fecal indicators for measuring the "risk". According to the WHO, Ecoli is the best. However, I found...
28 April 2016 7,938 6 View
How can I know if in a paper they are talking about gestational diabetes (the condition what starts during pregnancy) or gestational diabetes mellitus (women who had diabetes mellitus before being...
23 March 2015 4,424 7 View
I want to know the recommendation of energy contribution by meal in school children (9-11 years old). I have been reading that it is aprox 20-25% for breakfast, 25-30% for lunch, 20-25% for dinner...
07 October 2014 5,314 2 View
If I have a RCT where I have a control group (receiving funny data messages) and an intervention group (diet, physical activity tips reinforced with self-efficacy) with the aim of improving...
27 July 2014 9,228 10 View
I have a self-efficacy questionnaire and I want to know how can I classify the score of my questionnaire. Do I use the medium to classify the subjects into "self-efficacy" and "no-self efficacy"?...
26 February 2014 1,491 8 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View