Hi, recently the lab lost all the JM 109 competent cells of the Pgem T easy vector kit, so we found E. coli JM 1452 cells, Anyone knows something about this strain?
At the laboratory they are using a glycohemoglobin reagent set (Teco diagnostics), but I do not know how to "translate" those results into HbA1c values, so I can classify people into healthy...
06 March 2018 1,756 4 View
Dear colleagues. I'm trying to perform a WB to validate antibodies and I'm using Human ApoB100 (>95% SDS-PAGE) as a standard in the gel. I have tried two different loadings of protein 1.5 and 4...
31 December 2016 2,434 2 View
I have a self-efficacy questionnaire and I want to know how can I classify the score of my questionnaire. Do I use the medium to classify the subjects into "self-efficacy" and "no-self efficacy"?...
26 February 2014 1,452 8 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View