Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

How to estimate charge transfer and polarization resistance in a battery?

Hi. I have performed many EIS experiments using a cylindrical battery at different temperatures and state of charge. Some results (near SOC=0) are as the attached figure. How can I find the...

08 July 2019 1,746 2 View

Are there different types of Dansyl Chloride?

I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I...

24 June 2019 1,484 3 View

Exist some reliable website with information about seric calcium and phosphorus in Chelonoidis chilensis?

Someone will have papers, books or magazine articles about it. It's for my thesis. Thanks

03 June 2019 9,730 2 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

20 December 2018 3,718 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

11 December 2018 1,592 3 View

How to add different initial gas contents for different coal seams in a model with high dip angle in CMG GEM software?

28 October 2018 6,352 1 View

Loss of internal standard in a LC ESI MS/MS protocol for Gliclazide?

We are performing an LC ESI MS/MS protocol to quantify Gliclazide on plasma samples. We have noticed that the internal standard (Gliclazide D4) has a very high variation compared with gliclazide....

22 July 2018 8,936 4 View

How long the reflow lasts for the drying of the THF ?

I have refluxed the THF with sodium and benzophenone in an inert atmosphere, this solution turned yellow and should change to a blue color but I do not achieve that result, how long does it...

03 May 2018 4,182 4 View

What is half-life of mRNA of Tbet, AHR, and RORc?

01 February 2018 3,654 0 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View