Hey,
I'm currently preparing an experiment for a Cell Culture practical course in our University and am having some problems.
The students first synthesize Biopolymers in the first part of the practical course: particularly Polylactides, Polyglycolides in D, L, D/L-forms or copolymerized with NPC or Caprolactone. To solve the solids, we use Chloroform.
In the second part of the practical course, these Biopolymers are made into thin biofilms and are covered with HaCaT-cells to test the different growth rates on the different Biofilms, but it only works on glass plates, since Chloroform corrodes Polystyrol materials...
So my question, is there a chemical with both functions: to dissolve the Biopolymers and at the same time is compatible with Polystyrol ? Any experience ? Or should I just use glass ?
Glass seems to be your best shot, but you can use a mold or a surface of Teflon (Polytetrafluoroethylene), almost all the organic solvents will dissolve or deforme PS
Hey,
thanks for the answers. I talked with my Prof and the problem was that they need these special wellplates for the computer-assisted microscope that takes pictures every hour....so glass is no viable option.
Thanks, anyways
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