How to record RBC autoflourescence in a tissue sample?

RBCs have auto-fluorescence, I want to detect that auto-fluorescence in paraffin embedded tissue samples. Any suggestions?

08 February 2021 9,357 1 View

Why all samples are not detected by RT PCR ?

Hello everyone I did the RT PCR by using cyber green as a fluorescent dye however when I run the RT PCR of my two strains of Saccharomyces cerevisiae (duplicate) then I got the result in which the...

21 November 2020 2,076 3 View

Are there any thumbs up rules or procedures to convert your conference paper to an extended journal version?

Need to write an extended version of my work which is selected to publish in a journal.

28 May 2020 2,607 4 View

I need helper for interpretation of FTIR Graphs, anybody could help?

Can anybody help with interpretation of FTIR Graphs to read peaks of graphs for fruit bars made from fruits, herb and cereal?

18 May 2020 6,159 2 View

How to calculate error of a ratio of two data with errors?

Suppose I need to calculate A =(B/C), where B= 1.00+- 0.33 and C= 1.50+- 0.43 How do I calculate the error of A? as in, A=0.66+- ? Any idea if I can do this in excel or any other...

13 April 2020 7,667 3 View

I use NPS for drug detection the detection proved by UV, FTIR and AFM but DLS shows a abrupt result how can i interpret the results?

after the interaction of drug the enhancement in the SPR band was observed the intewraction was proved by FTIR and AFM the AFM resiults shows aggregation of NPs the of NPS increases from 40nm to...

10 February 2020 9,355 3 View

Aggregation of nanoparticles leads to large shift in the SPR band but in my case only broadening and quenching in absorption band was observed why?

its colorimetric detection after interacting a analye with nanoparticles color rapidly change from yellow to orange. after 15 min of interaction take of a absorption spectra only quenching...

04 November 2019 7,712 1 View

I want to do protein expression in E. faecalis JH22 by using anhydrotetracycline (ATc) to induce protein expression .Need protocol for expression?

I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.

26 July 2019 1,979 2 View

How to peel off simple dried chitosan film (from glass or plastic/substrate) without neutralization (NaOH/KOH)?

I am trying to make chitosan film (of various concentrations)on glass substrate by simple casting method from solution in acetic acid. After the solution dries, film sticks to glass so firmly that...

11 June 2019 7,504 3 View

Cost-effectiveness model: Converting different rates on different rates into one probability?

Hello Everyone, Need quick help! I have 5 different rates for recurrence from 5 studies with different followup. How do I combine them to find one probability to be used in cost-effectiveness...

05 November 2017 4,107 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View