Hi,
I saw some publications that recommended Neon transfection system, but I don't have it. I do have BTX and AMAXA transfection systems.
Have these transfection systems worked for you?
It might be best to stick to a transfection protocol and system commonly used in research that you are trying to add to as preliminary work to minimize differences in methods (generally). But, sometimes, the differences of these systems are just a matter of branding different devices. And, since you are dealing with a cell line, there shouldn't be a problem with trying multiple systems and looking for cell viabilities unless there is a budget issue or some other constraint.
Good luck! Hope this helps!
As long as you have a good buffer for Jurkat cells (https://altogen.com/product/jurkat-electroporation-kit-t-cell-leukemia-tib-152/) any electroporation system should suffice. Given that jurkat is a fairly popular cell line, it's likely that there are electroporation protocols published in the literature. It will obviously take some experimentation, and setting up a good pre-testing procedure will help with results down the line.
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