Does anyone know of a publication that has data on increased extracellular serotonin levels directly after SSRI administration? More specifically, do what the difference in 5-HT levels are in participants with the long allele in the 5-HTT promoter region vs participants with the short allele. Mice models will also work with 5-HTT knockout mice (+/+ vs +/- genotype).
HI Pettitt,
You can measure the extracellular level of 5-HT by using microdialysis. Take a look at this paper. Samples can be collected at different intervals and analyzed after administration of any drug i.e. SSRI to see the effect on extracellular concentration of the 5-HT.
http://www.ncbi.nlm.nih.gov/pubmed/21537332
People in Marc Caron's lab at Duke University have expertise in doing that, so you might wanna get in touch with Jacob to know more about the same.
Hope this help.
good luck
Hello,
we did a lot of work on the effect of SSRIs on extracellular serotonin. For example Ceglia et al 2004 (British J Pharmacol 142: 469) describes the acute and chronic effects of escitalopram on extracellular serotonin in the rat prefrontal cortex. You can do a pubmed search on francesc Artigas papers who also wrote excellent reviews. Look also at Shen et al 2004 (Neuropsychopharmacology 29: 1790 and Mathews et al 2004 (J Neurosci Meth 140: 169) for SERT ko mice.
Hope this may be useful
BR
William
Hi Pettitt
5-HT and 5-HIAA levels during in vivo brain microdialysis may be reflected to the difference status in sham-operated and the other animals model rats. 5-HT levels were similar in both groups of rats; 5-HIAA levels were higher in rat model of disease compared with sham-operated control rats.
We investigated the role of serotonergic systems on the fatigue induced to cholestasis in bile duct–resected rats. This is a summary related to part of serotonin in my paper, I hope, this can help you.
"5-HT and 5-HIAA measurements in the hypothalamic dialysis perfusion fluid were preferred over tissue measurements, as extracellular measurements are known to reflect the functional status of the neurotransmitter more accurately avoiding the influence from other compartments, such as nerve endings and endothelial vessels. Tissue measurements, on the other hand, represent a mixture of intracellular and extracellular content. The results of in vivo microdialysis experiments indicate that central extracellular 5-HT levels are similar in rats with BDR and sham-operated control rats, whereas levels of 5-HIAA are increased in rats with BDR compared with sham-operated control rats. These results suggest that 5-HT metabolism is increased in rats with BDR with essentially unchanged basal 5-HT output, similar to the situation in more severe liver disease with hepatic 5-HT uptake compensates for the change in release—thus maintaining the extracellular level at a more or less constant level cannot be ruled out. Overall, these findings argue against a functional overactivity of the serotoninergic system as a potential cause of the fatigue associated with cholestasis.
On the other hand, these data can be reconciled with decreased presynaptic 5-HT1A activity, because activation of 5-HT1A presynaptic receptors leads to decreased 5-HIAA levels"
Turgay celik
Thank you everyone for your answers, they were exactly what I was looking for!
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