We are interested in separating both DNA and RNA from some precious frozen tissue. We have standardized the procedure of DNA separation with GenElute™ Mammalian Genomic DNA Miniprep Kit from SIGMA (code G1N10). I was wondering if we may also try to recover RNA from the liquid we usually discard (of course avoiding RNAse step) or if we are forced to change our SOP and move to specific kit? Thanks.
I don't if it will work, but you can try to use Amicon® Ultra. It's used for purification and concentration of DNA/RNA /proteins so if you separate DNA before, it's probable that that might work.
I doubt it will work. The binding to spin filter membranes in chaotropic salts is non specific for DNA and RNA - both will bind which is often why you have to add an RNAse step
You can use TriReagent (sigma) and back extract the DNA from the interface.
The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on
Nature Protocols 1, - 581 - 585 (2006)
Piotr Chomczynski1 & Nicoletta Sacchi2.
RNAzol® BD: a reagent for the effective isolation of RNA from whole blood
Piotr Chomczynski,
William Wilfinger,
Amy Kennedy,
Michal Rymaszewski
& Karol Mackey
Nature Methods 10(2013)
Ian Teo
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