Agarose EMSA for aptamer binding: no bound complex band?

Hey there, Due to limited resource, we are trying to assess the interaction of a 50mer aptamer with a 25kDa protein domain. The protocol we are using is unusual in that the gel is 2.5% agarose...

23 July 2020 8,192 8 View

Design of PCR primers for extremely short oligonucleotides?

Hello there, hoping you are well, I have reached a point in my current project where I need to amplify very very short (50mer) non-coding ssDNAs. I understand the options I have available...

20 July 2020 1,558 5 View

Can the overall similarity of a standard mass spectrum to that from an unknown be used for identification without using the fragmentation ion ratio?

Here is situation: Compound is run on GCMS give a mass spectrum. Old standards are used from a library. The identification is made by observing with eye that the two OVERALL patterns are...

11 February 2020 3,965 3 View

What operations are necessary for a proper identification of a compound using optical microscopic methods?

It is my understanding that the forensic identification of a controlled substance using optical microscopy requires more information that just the classification of the type of crystal formed....

27 January 2020 5,751 2 View

What software is available to model the impact of design change in complex products?

This question relates to research into the management of engineering design information and the impact of innovation. Complex products such as aircraft, trains and ships are high-value, long-life...

18 December 2019 316 3 View

What software is available to model the impact of design change in complex products?

Complex products such as aircraft, trains and ships are high-value, long-life assets that are typically managed as a fleet over a period of 20 to 30 years. Managing technology upgrades...

18 December 2019 4,710 3 View

cannot delete question?

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27 April 2018 5,230 4 View

In SPSS, how can I adjust data to remove the effect of a covariate?

In brief overview, I have grown some plants and measured plant height (amongst many other traits), but my plants have been damaged by frost. I gave each plant a frost score between 0-5 as a...

05 March 2018 5,520 4 View

Haemoxygenase-1: does anyone have expertise for what solvent to use to store this protein in at -80 degrees?

Hi We recently started doing some ELISAs to look for levels of this protein, but are finding that reconstitution in water (as instructed by manufacturer) probably didn't completely solubilise the...

09 May 2017 7,815 1 View

What computational advantages does computing a feasible solution have compared to computing an optimal solution?

Under the same conditions of previous question

11 December 2015 5,915 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

What is India's InternationalTrade Policy during Pendamic? How do you find the policy as successful one?

During Covid-19 Pendamic Government are facing problem to maintain business. India is one of them so this question is perfect to be discussed here.

08 February 2021 2,725 3 View

I am struggling with the concept of preprint papers and wonder how people feel about them?

Specifically, how should we use them given they have not been peer-reviewed? Should we rely on the results reported in pre-prints for policy? I am really probing for thoughts on whether...

04 February 2021 3,175 15 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What is the role of IQAC to improve the grading of an institution?

IQAC provides all information about institution and its various activities to NAAC, Bangluru, India in order to get good grading. In this light what is the role of IQAC??

03 February 2021 6,916 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Papers/studies on public policy and translation/implementation of global policy?

Hi all, I am looking for papers/books/studies related to changes in public policy, including theoretical papers, and on the translation/implementation of global policy onto the national level....

25 January 2021 2,242 7 View