It's interesting that it counts optimal conditions for IEF based on a salt concetration. It can save a lot of time when you have a new sample.
Are there any better possibilities to manage that?
That's kinda clever. Personally I use a Horiba 173 conductivity meter. It's designed for water testing, but it has a slot that hold 50uL for measurement and you can get the sample back. Anything below 200uS is OK in my expereince, the lower the better. In their example they wouldn't focus the sample reading 111uS which I think is being conservative. Anyway, it takes 10 mins with a BioSpin equilibrated in 7M urea, 2M thiourea to remove salt. What some people don't realise is that solutions of UT can have high conductivity and need to be desalted with a mixed mode ion exchange resin before using for sample prep and focusing.
As for focusing time, I focus O/N to at least 100kVh using 11cm strips, sometimes longer. The focusing is fine. 24cm strips I've focused to 300kVh to get the resolution.
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