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Questions related from Jan Gebauer
I was quite disappointed by low specificity of Ni-NTA for binding His-tagged proteins. Many other proteins have affinity to nickel because of the metal-binding motifs in their structure. Any...
11 November 2013 5,521 6 View
I have a sample from immunoprecipitation column - polyclonal porcine antibodies in complexes with salmonella proteins (all in PBS). Does anyone have suggestion about how to separate them and...
10 October 2012 5,481 16 View
It's interesting that it counts optimal conditions for IEF based on a salt concetration. It can save a lot of time when you have a new sample. Are there any better possibilities to manage that?
10 October 2012 9,732 2 View
