Do you have fiber probe? If not, use a plastic bag, take the spectra of it, fill it with water and you can monitor the spectra of the water in the back in both ways: the freezing and the melting process after subtracting the spectra of the plastic bag.

Peter, aquaphotomics can tell you how much info is there in the water spectrum. enjoy it!

Hi Jelena,

I thin that all has been said. Now, why to choose a so short pathlength for the spectrum?

Thank you all for answers, very kind of you :)

I have an idea what to do now, thank you prof. Tsenkova. 

@ R.Tsenkova: I don't have a probe, unfortunately, but I will try to do with a customized holder and a plastic wrap. I may even be able to remove the wrap after freezing...I'll see how it goes. 

@ A.K. Kuptsov: Thank you for this interesting idea..I think I can do this with low-e microscopic slides, although I'll try first with prof. Tsenkova setup, it is easier. 

@ J. GE Thierie: I am interested in entire region from UV to NIR and with the instrument I am using, even this pathlength is too much, the combination band of water is too strong and over saturated. It is even recommended using of additional magnetic screens when working with strong absorbers, to adjust the reference beam. 

@ P. Kapusta - well, there are some things not very clear to me, about so called exclusion zone water which supposedly absorbs in UV region, for example you can see it in this paper:

http://agelesssecret.com/wp-content/uploads/2014/03/Highlight-WATER.2014.6.Dibble.pdf

So, I wanted to see some things for myself and compare it with the water bands in NIR region  :) As prof. Tsenkova said, lots of new things can be found with Aquaphotomics :)

Jelena,

I also observed an "aura" effect in water but in the NIR region. According to Jerry Pollack, who saw the photo, it would be an EZ effect.

You can read my article on ResearchGate:

  "Short communication: Near infrared dynamic measurements of activated sludge settling highlight the possibility of a local modification of free water properties."

If you are interested, I can send you the photo of the aura I observed at 940 nm.

Jacques

Do not see that 1 mm would be necessary instead of 10 mm. Me to would expect only change in NIR. Cell made of PE or PP would be more flexible on freezing. Whether cell breaks or not will largely depend on speed of cooling and on temperature. If you slowly go from 1°C to -1°C likely nothing will happen. Range below 0 allowed can be only found out by experiment unfortunately. Would do slow freezing outside the instrument and then let the frozen water slowly melt inside the instrument.