Does anyone have a protocol for the extraction of lignin content from soybean seed coat using spectrophotometer?

More Dr Sanjay Pathare's questions See All

Are you looking for research collaboration ?

we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]

29 July 2024 6,626 0 View

Dear researchers. pl help how to plot jablonski energy level graph and magnetic hysteresis curve in origin?

through origin software

17 July 2024 4,991 0 View

How is artificial intelligence being utilized to enhance the diagnosis and treatment of sleep apnea?

AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.

03 July 2024 9,393 2 View

Action research design is Quantitative Studies or Qualitative Studies?

14 June 2024 1,684 1 View

Offer or collaboration for book publications?

Dear Profs/ Scientist, if any one is interested or have plan to publish a good quality books/ book edited , I can contribute/help such type of research works . My research...

06 June 2024 5,932 2 View

How to draw SeDeM diagram for preformulation study of drug and excipients?

What is SeDeM expert tool? Is it in silico tool and how to draw the SeDeM diagram for preformulation study? Kindly suggest.

06 June 2024 4,408 0 View

Call of book chapter ?

CALL FOR BOOK CHAPTERS (No Publication Fee) Scrivener Publishing/wiley Dear Prof,Scientist/ experts, Greetings. We are editing the book entitled " FET Devices: Post CMOS Theory and Applications...

28 May 2024 9,284 0 View

Is there a Raising awareness of indigenous medicine among metropolitans?

The growing interest in holistic health practices is leading to increased awareness of indigenous medicine among metropolitan populations. While challenges exist in terms of access and cultural...

09 May 2024 7,041 1 View

Why many times RG is sending email? Is this person is co author or published article? Many times it is been sending.?

Pls dont send many times email is this person is co author or published article? Many times it is been sending. Pls stop it ok

06 May 2024 3,964 3 View

What is the protocol to maintain 260/280 and 260/230 and to get pure DNA from ruminant faeces using Dneasy PowerSoil pro Kit?

I used the Dneasy PowerSoil Pro Kit. But I didn't get a good ratio of 260/280 and 260/230 and didn't get a good band after electrophoresis. I maintain all procedures according to the...

29 April 2024 1,242 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Marta Nunes da Silva

Hello. :) Here is an acetyl-bromide method adapted from Fukushima & Hatfield frequently done in our lab. I hope it helps. ;)

Dr Sanjay Pathare

Thank you Very much. it could be very helpful for me.

Dyoni M. Oliveira

In addition, you can test this another methodology for acetyl bromide method.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110000