❓ Full Question:

I am currently working on measuring antioxidant enzyme activity—specifically Superoxide Dismutase (SOD) and Catalase (CAT)—from blood samples using spectrophotometric methods.

I am looking for standardized, peer-reviewed protocols or personal lab-validated methods that have been used successfully in experimental or clinical research.

🔍 Specific Information Needed:

📚 Background:

• I am using whole blood samples from human subjects.
• Available equipment includes a UV-Vis spectrophotometer (200–700 nm range).
• I aim to report enzyme activity in U/mL or U/mg protein.

🧬 Relevant Tags:

Antioxidant Assays SOD Assay Catalase Assay Spectrophotometry Blood Biochemistry Enzyme Kinetics Oxidative Stress