That question makes me think of M Bouaboula - a much cited and revered resource for me through my PhD. If you see below there should be a reference to a paper with binding details in (cell line binding) from this group, and some detail from said publication.
Modulation of CB1 Cannabinoid Receptor Functions after a
Long-Term Exposure to Agonist or Inverse Agonist in the
Chinese Hamster Ovary Cell Expression System (Rinaldi-Carmona, M et al)
Binding experiments. CHO-CB1 cells were washed and incubated at 37°C with
[3H]-CP 55,940 (0.1 to 30 nM) or [3H]-SR 141716A (0.3 to 30 nM) in
0.5 ml of binding buffer [25 mM HEPES/Tris (pH 7.5), 140 mM NaCl,
5 mM KCl, 1.8 mM CaCl2, 0.9 mM MgCl2, 4.5 g/l glucose and 0.2%
acid-free BSA] for 30 min. Rapid aspiration of the assay medium
stopped the reaction. Cells were then rinsed (three times) with 1 ml
of binding buffer. After addition of 0.1 M NaOH (2 3 0.5 ml), extracts
were transferred and the radioactivity bound to the cells was counted
with 4 ml of biofluor liquid scintillant. Nonspecific binding was
determined in the presence of 1 mM of their respective unlabeled
ligand.
Look for our work on medline search and you will find several of our papers on this topic. Hungund BL and Associates, Basavarajappa BS and also Vinod KY or Vinod Yaragudri.
As Mr. Banister, I'm also interested in a similar protocolo for CB2 receptor, which is the correct/best antibody to perform a western-blot analysis for detecting CB2 receptors?
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