We study interaction of physcomitrella patens with plant pathogenic bacteria and found high level of plant reaction diversity to some strains in different experiments. Is it a result of somaclonal variation, age-depended reaction or anything else?
Thank you, Dr. Khanizadeh. Phenolics in the moss is one of our ideas to solve the problem.
Hi, Alex, have you used agar or gerlite? Agar may be the reason of variation. Even small variation in growth conditions (ea. position of the pates in the shelf) have a strong effect on many physooligical process, including, of course , sensitivity to pathogen. Good luck!
Taras, thank you! We had variation in liquid medium (a full range from salts solution only to full growth medium) with protonema reaction. it was OK within one-two experiments (4 replications), but could be changed to opposite reaction in next one.
Thanks, Alex, to overcome variation problem I would suggest you the following way: prepare once time stock (you can use x2 times more concentrated) for whole set of experiments, freeze it at -20 C and always use filter sterilization before experiments. Of course, the best decision is to make 3-4 repetitions one by one form the same medium, same cells and during short time period (3-4 days). Good luck!
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