Try the available broad spectrum antibiotics in the country. If not, find antibiotics that work on protein synthesis. Copper sulfate works also but, try different concentrations. If not, come back to me. On the experimental level, 5,fluorouracil is the best.

Thank you, Mohamed. Antibiotics are not permitted for use in agriculture in Russia. Anyway, this Agrobacterium is resistant to 90% of them. We are testing copper, antogonistic Bacillus and phages.

Although I have no experiences with substrate I think our biological system of soil desinfestation ( www.bodemresetten.nl) should have a good chance getting in control with these bacteria. In Holland we are on the market controlling soilborne diseases since 2009. Even in the biological segment we are performing good.

In july 2014 our system will be discussed in Torino (www.sd2014.org) by presentation with a killing rate of > 99.5 % of potato cyst nematode. Brownrot, Ehec bacteria and synchitrium endoboiticum are also examined otherwise upto killing levels up to 100% So sunbstrate trearment is e new approach but maybe it can work also.

Alex. From memory there were two incidences in the UK where root mat disappeared. These were both times when crops switched to a soil based system and therefore suggests some form of competition effect took over. It was always my feeling that some form if biocontrol was the only 

Simon, thank you! I have a strong feeling that the initial outbreak of 1993 could be a result of glasshouse experiments with cucumber and "A. rhizogenes" strains isolated at 1970-s. Agrobacterium bv1 in Russia survives in rivers water, so, it may be not competitive in soil, just like in UK. We found it in fruits and mature seeds obtained from  the diseased plants.

Alex. Have you had any success it controlling the disease? I am interested it is still a problem. Simon

Simon, my colleagues apply iodine with PEG (FarmaIod) for irrigation system and substrate disinfection + Actinomyces-based bio-control agent (Fitolavin-300) with some success. We are testing plants for some genetic resistance - a few varieties were tolerant to this pathogen.

I found a beautiful experimental paper about root mat in Japan:

SAWADA, H.1 and AZEGAMI, K.2 (2014). First report of root mat (hairy root) of tomato (Lycopersicon esculentum) caused by Rhizobium radiobacter harboring cucumopine Ri plasmid in Japan. Jpn. J. Phytopathol. 80: 98–114.

They have got the disease for the first time at 2011.  Infected seeds?

Simon A. Weller Have you ever met "crazy roots" on outdoor plants? We have suspected symptoms on carrot, physalis and apple tree.

Alex Ignatov, no only cucumber and tomato in my experience. I would be interested to know if rhizogenic agrobacterium were the causative agents of the symptoms on those species

Simon A. Weller Simon I will bring the samples to Lab for analysis. So far, you can see the pictures https://www.researchgate.net/post/Root_proliferation_on_3-years_old_tree_is_this_crazy_roots_form_new_for_apple

Hi,

have you read this material? Might be useful:

https://horticulture.ahdb.org.uk/sites/default/files/research_papers/PE%20029_Review%20document%20%2810June16%29.pdf

https://www.actahort.org/members/showpdf?booknrarnr=1237_1

https://horticulture.ahdb.org.uk/sites/default/files/research_papers/PE%20029_Report_Final_2018.pdf

https://www.sciencedirect.com/science/article/pii/S0734975019300953

https://link.springer.com/article/10.1007/s00253-019-10003-0

https://www.koppertus.com/challenges/disease-control/crazy-roots-hairy-roots-crown-gall/

https://www.hortidaily.com/article/45240/Enriched-biochar-reduces-severity-of-root-mat-by-half-in-AHDB-trial/

For the outdoor samples you are showing I have a question: did you check for nematodes? Just wondering.

Francesco Desiderio Yes, there is no nematodes, except free-living. Thank you for references!

I especially like sentence from ADAS Report of 2018 ( Protected tomato: Evaluation of biological treatments, biocides and an improved diagnostic for control of root mat disease )- A newly formulated sodium hypochlorite product (Domestos Extended Power) was the only product tested that eliminated viable R. radiobacter in biofilms on irrigation tubing within the experimental conditions used.

and : "Mutation breeding, chromosome doubling and introduction of rol-genes from Agrobacterium rhizogenes in plants are interesting alternative breeding approaches. https://www.actahort.org/members/showpdf?booknrarnr=1237_1

Could it be an incompatible interaction, what is observed? As far as I know there are some root nodules in Leguminosae which has a sort of symbiosis, in some cases, but might not work for cucumber, tomato and other species, which are far from N fixators...

Here is some other material:

http://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_53-54/010017170.pdf

Article Colonization of Phaseolus vulgaris nodules by Agrobacterium ...

Article Rhizogenic Agrobacterium biovar 1 and cucumber root mat in the UK