How to make sure that ultrasonic lysis of E.coli is done completely?

What's the criteria of comolete lysis of E.coli when breaking them using ultrasonic? What phenomena can help to determine if the cell are broken completely? From smell, transparence of the lysis...

07 January 2021 1,911 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Ungrounded CPW fed slot-ring patch antenna not behaving as an antenna in CST simulation?

Greetings everyone, I am attempting to model and simulate a slot-ring patch antenna fed by ungrounded CPW in CST, although the S11 is fine, the e-field and h-field monitors shows strange...

10 October 2020 2,228 2 View

Can we perform MOX/MSTFA derivatization in Eppendorf tubes?

Hi there! Recently I am planning to work on metabolomics using GC-MS, where derivatization is a crucial step. Here, I am wondering if that possible to derivatize extract samples with both MOX and...

24 September 2020 9,695 1 View

What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to eliminate the warning of 'position not found when parsing line' during DAMASK simulation with ABAQUS/standard?

Hi, I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw...

10 December 2019 4,667 1 View

What is the yellow/brown salt crystal during protein crystallization?

During protein crystallization screening, we encountered several types of proteins grew yellow stick crystals with a reservoir containing 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate. However,...

09 January 2019 2,188 1 View

What is a quick and easy screen for visualizing the presence of gold nanoparticles?

Hi all, I am trying to design a hypothetical screen to find additional genes that are responsible for C. metallidurans' ability to reduce gold ions (Au(I) and Au(III)) to solid gold nanoparticles....

12 February 2018 7,511 2 View

One virus may have two different kinds of particle?

15 February 2017 676 10 View

Genotyping PCR works for adult mice, but not for embryos?

We genotype adult and fetal mice routinely in our lab. PCR always works smoothly for adults, but the same primers would not produce bands consistently for embryos. As shown in the attachment, the...

14 September 2016 8,154 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

How to differentiate the economic productivity in agriculture and in manufacturing sector?

Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...

15 February 2021 8,342 7 View

How could I do a simulation for the energy consumption of a CNC machine?

I'm working on my dissertation and it is about how to save energy when a CNC machine goes to idle mode. (A concept model of the idle machine energy saving in industrial manufacturing...

26 January 2021 9,620 5 View

How to add a single parameter in Ansys Additive Manufacturing Workbench parameter setting dialogue box?

Ansys 19.2 R2 and 2020 R1 versions of Additive Manufacturing Workbench is convenient for powder-based Additive Manufacturing processing. However, in this workbench, there is a machine parameter...

19 January 2021 3,017 2 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

What is your expert opinion/experience/suggestion about the new variant of Novel Corona Virus (COVID-19)?

Dear Research Gate Members, Nowadays, everyone is talking about the new variant of Corona virus COVID-19 and its affects are alarming throughout the world. Please, share your expert suggestions...

29 December 2020 7,555 20 View

Have you observed the improvements in Online Meeting Platforms during COVID-19? In this context, how will you compare Google ZOOM & Microsoft TEAMS?

Dear Research Gate Members, Online Education / Distance Learning and Work From Home (WFH) are the ultimate new realities for many in the world due to Pandemic Novel Corona Virus (COVID-19)...

29 December 2020 9,866 37 View

Hello everyone, which software is preferable for simulating the effect of shrinkage of metal powders for the Additive manufacturing process?

Last time I was asked about simulation software to analyze the effect of shrinkages of plastic powder materials. Currently, an additive manufacturing process is what I find suitable for my...

29 December 2020 4,615 9 View

How China is the biggest emitter of CO2 gas in the world?

How China is the biggest emitter of CO2 gas in the world?

25 December 2020 6,184 10 View