Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Does anyone have a qPCR protocol for Lactuca Sativa?

I need to extract RNA, produce cDNA and do my measures via qPCR. How much material? Which stage of growth?

24 January 2021 559 2 View

What fluorescent dye can be used in fixed cells for membrane imaging?

07 January 2021 4,802 2 View

Morphogeometrics analyses: how do I know which landmark has the greatest variance?

I'm using MorphoJ, and the software gives me the eigen values but it doesn't give you the eigen vector. For each landmark it gives you a x and y value for each PC but I'm looking for a value that...

21 December 2020 3,466 3 View

How to calculate Dose rate \dot {D} without source activity with dose tally * F8?

I am using MCNP5 to calculate the dose in a Brachytherapy source with dose counter * F8, but I do not have the current activity of the source. How can I calculate the dose rate for this? Thanks...

06 November 2020 4,194 3 View

Methods for bird trend analysis?

What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...

27 October 2020 7,532 5 View

What do you think about social exclusion?

The social exclusion is a problem very important for society, we should all help those most need

12 March 2020 1,152 3 View

PJET ligation not working?

I am trying to clone a 1.3 kb fragment using the pJet system. It is a sticky end cloning protocol and I have tried different ratios (from 3:1 to 8:1). I have used DH5a and Top10 cells with...

18 February 2020 9,178 3 View

How can I normalize Western blots if housekeeping proteins change in time?

Hello, I am evaluating the expression pattern of some proteins during the process of neuronal differentiation (1 day in vitro to 32 days in vitro) in primary neuron culture using western blot. I...

08 September 2019 1,505 3 View

How to extend the life shell of a biofertilizer?

Hi I am currently working in a project to develop a biofertilizer but my main problem is that my product only last 1-2 weeks and then it gets rotten. Is there any protocol or conservative sustance...

27 January 2019 9,096 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Do you have papers on hindlimbs myology of birds to share?

Hi all! My work team and I are looking for papers on muscular descriptions of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than...

22 February 2021 8,330 6 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View