We got thermal hyperalgesia, after 0.1 mg for two weeks or 0.5 mg for 5 days. But we could not find mechanical hyperalgesia in these treatments. How can one induce neuropathy, quantifiably through Von frey filaments?
Hello Khalid,
without more detail regarding your study it is difficult to speculate where the issue is, but off the top of my head there could be three main reasons why you struggle to measure mechanical hypersensitivity.
1. The mouse strain you are using. Development of mechanical hypersensitivity can vary considerably between strains. I am aware of three strains that have been used to measure mechanical hypersensitivity in mice: ICR, Balb/c and C57Bl/6J.
2. Toxicity of vincristine. Due to the toxicity of vincristine complications including motor deficits can occur which would make behavioural testing difficult. You could check if your animals show any motor impairment or double check their body weight to evaluate their general wellbeing. Furthermore, I don't know which vincristine protocol you are using exactly, but you could consider to have a schedule with breaks in between the vincristine treatments minimise these side effects.
3. Your method of measuring mechanical hypersensitivity. In general I would advise to use a manual method of measuring this in mice. The up and down method has been used to measure vincristine-induced hypersensitivity, but also measurement of %responses to a set of von-Frey hairs.
Best,
Rachel
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