We have project concerned to biomechanical testing of fresh pig bones that are glued together. Bone adhesive that we are using has setting time 72 hours. During 72 hours fresh bones in temperature 37° start to be under putrefaction. We are interested how to stop or to slow down this process.
When testing fresh bone several days after harvesting, one can store specimens in gentamicin-doped saline to prevent putrefaction. The question is then whether the glue you are using will cure in water. If not you will need to devise a way to isolate the glued regions. Alternatively, there are some epoxies that will cure while submerged, including slow-curing varieties that will not produce much heat and will thus not damage the bone.
- Dear David, thank you very much for your helpful answer. Our newly developed bone adhesive works well in water. Idea with gentamicin doped saline should help us :-) Pleas could you write me how to prepare it, especially how should be concentration of gentamicine in the saline? Thank you for your answer.
I am not certain of the ideal concentration, but there is a lot of published research on delivery of gentamicin in vivo and in vitro with respect to bone grafts and you can probably find some useful information there. The following is available on RG:
Naraharisetti, P.K., Lew, M.D.N., Fu, Y.C., Lee, D.J. and Wang, C.H., 2005. Gentamicin-loaded discs and microspheres and their modifications: characterization and in vitro release. Journal of controlled release, 102(2), pp.345-359.
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