How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Could you please share your experience in learning SPSS?

I would like to learn SPSS. Could you please guide me or share your experience if you feel it would help me to learn SPSS? Thank you very much indeed!

08 November 2020 7,357 6 View

How do I compare time domain solution with frequency domain?

I am solving EM wave scattering by PEC cylinder numerically and wanted to compare the scattering width. My result is in the time domain which is evolving with time but the analytical solution is...

11 October 2020 770 3 View

Is it possible to consider intermittency of renewable energy sources by using normally (Gaussian) distributed random numbers ?

Consider wind speed for wind power and solar radiance for solar power. Is it possible to consider intermittency of mentioned sources using normally ( Gaussian ) distributed random numbers in which...

29 January 2020 5,731 3 View

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

12 January 2020 9,036 3 View

How to prepare 1U of lyophilized powder of Bacillus stearothermophilus?

First of all, i have narrow knowledge about antidiabetic assay due to this is my first enzymatic test and i have no one to refer. I have a method but when i tried to google every each of the...

05 January 2020 4,629 3 View

How to compute unknown parameters in an equation from experimental data?

Is there a way to compute some unknown parameters in an equation using curve fitting using experimental data? I am upload a picture which contains the function and three unknown parameters and...

25 December 2019 2,184 7 View

From the Table attached, why Retentivity is decreased for Bi:ZnO 3% and for Bi:ZnO 5%, Coercivity is decreased but Ms is increasing linearly for all ?

Hi all, referring to the table attached, it is seen that when ZnO NWs are doped with Bi at different concentrations, there is an increase in magnetization values initially. But we see by doping...

08 October 2019 3,938 3 View

How to make 500 unit/ml catalase solution using catalase protein from Sigma?

13 August 2019 7,931 3 View

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10 March 2019 8,910 11 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Will the of use of vermicompost as nutrient source in agronomic crops possible ?

We know that vermicompost is far better than compost manure and chemical is too exe and deficit too so for marginalized farmer it will be better to promote vermicompost but its production in...

09 December 2020 1,873 22 View

How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

Can anyone help with SDS-PAGE problems?

i'm working on protein evaluation in flour of wheat and others legumes.....I'm using SDS-PAGE. However those days (about two weeks ago) i foudd nothing in the gels (no bands), i dont know what's...

11 November 2020 2,330 3 View

Which nutritional component of grains affect the pH level?

Dear community I have a great doubt related to pH in grains. It has been reported that some amino acids could be influencing the pH level. However, could minerals located in the inner parts of...

24 September 2020 6,694 14 View

How to sample maize leaves and transporting them for specific leaf area measurements?

Dear RG community, I would like to sample (cut-off) maize leaves in the field and transport them to the lab for SLA (specific leaf area) measurements. After a brief literature review, I could not...

18 August 2020 6,079 4 View

Can anyone please suggest me how to convert gene ids to gene names of maize?

I have a tabular file containing two columns of gene id and expression counts, I need to convert gene ids to gene name by software? I can't do it manually by going to NCBI as I have a large number...

03 August 2020 3,512 3 View

Why organic fertilizers are mostly more effective in light texture soils (sandy and loam) than heavy textural soils (clay)?

In my recent study, I have found a pronounced effect of vermicompost on increasing grain yield of maize on sandy and loam textural soils as compared to clay textural soils.

01 June 2020 6,098 13 View