Hi all, I am preparing antibody functionalized carbon nanotube in suspension through EDC/NHS chemistry. Briefly, I am activating the -COOH group attached to my SWNT by EDC/NHS in MES buffer and I am starting to see agglomeration of the CNT in the solution. The CNTs are dispersed but in bundled/agglomerated form. After centrifugation and washing and subsequent addition of antibody, I am experiencing slightly larger agglomeration of the CNT, which if kept at rest, settles down. I haven't used any surfactant (like tween 20 or Triton-x) yet. Is this normal to observe this kind of situation while functionalizing CNT in solution/ suspension? Thanks.
Edc/nhs renders the system unstable due to charge suppression. Adding the antibody simultaneously might help. Edc/nhs sucks.
When CNTs are funtionalized their zeta potential change and they could agglomarate or precipitate. If you require your material disperse in solution you can test it in different pHs.
Marlen Alexis González-Reyna
Thanks for the suggestion. But the activation by EDC NHS requires it to be pH 5~6. So if I change it, it might interfere with the activity. Is there any other way of dispersing? Or is it okay to work with this suspension in slightly agglomerated condition? Thanks.
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