Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Can Qualtrics generate reports for survey respondents that show their answers to selected questions?

I am using Qualtrics for a Delphi survey. Does anyone know if Qualtrics can generate reports that I can send to respondents. More specifically, I want to send respondents a report of their...

13 January 2021 7,976 3 View

What are health and agricultural econmic impact of the herbicide 2,4-dichlorophenoxyacetic_acid (2,4-D) in your country?

Dear Research community, I am writing a review about 2,4-D. Google Scholar has identified 11000 publications with this herbicide as a keyword since 01/01/2018. There is plenty for me to read....

29 December 2020 1,145 6 View

What are the ethical considerations when conducting research with students who are paying for a private course?

I'm going to be teaching an online course to minors using game-based learning strategies. I wasn't to make sure I consider ethics considering students (likely their parents) will be paying to take...

12 December 2020 6,497 11 View

No host reference for metatranscriptomics?

One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...

30 November 2020 3,259 2 View

Primer specificity check with primer blast individually or together?

Hi, When checking my designed primers for specificity, what is the difference if I run BLASTn for each primer individually versus together (ie. forward primer separated by 20 "N" then reverse...

03 November 2020 1,421 4 View

To what extent do motorhomes and other large vehicles degrade road surfaces in relation to cars and other light vehicles?

I am studying the effects on the road system of a rural region of a drastic increase in traffic, particularly Motorhomes. My question is this, do motorhomes and other large vehicles have a...

22 October 2020 8,146 3 View

How to calculate pKa's using DFT for a molecule with three acidic hydrogens?

The molecule in question is a molecule with 3 non-equivalent hydrogens. Am I going to have to run the calculations in all of the transition states?

18 October 2020 1,524 3 View

Gibson Assembly Produces Wrong Product?

I have been using Gibson Assembly to insert a 7kB fragment into an 8kB linearized vector. After assembly many colonies form, but colony PCR on the transformation consistently shows a 1.5 kB...

18 September 2020 247 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View