17 January 2019 3 4K Report

Do you know the risk of Neuroblastoma ?

More Francesco L. Giusepe's questions See All
How to determine the charge passing during a cyclic voltammetry?

Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...

29 July 2024 3,733 5 View

Differences between Surface Tension and Interfacial Tension in Liquid-Gas Phases?

Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...

18 July 2024 382 5 View

EIS spectra analysis software?

Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...

10 July 2024 8,880 4 View

To what extent Google Scholars Citations system is reliable?

1. I was shocked to discover yesterday that my research citations on Google Scholar had dropped to 2383 from 2419 a week ago, and they are now up to 2391. 2. My citations three months ago were...

28 June 2024 2,846 2 View

How to perform a double step optimization with ORCA?

Hello to everyone, I am trying to launch a BLYP optimization with ORCA starting from an already converged calculation at BP level ,exploiting its orbitals and geometry as a guess. I tried...

24 June 2024 3,768 2 View

Is a binary classifier based on Gaussian models resistant to the problem of training set imbalance?

A binary classifier based on multivariate Gaussian models, which estimates the mean vector and the variance-covariance matrix during the training phase and returns the class with the highest...

23 June 2024 10,114 1 View

How to find out hysteretic energy consumption of the pier after earthquake excitation in sap?

how to observe the hysteretic energy consumption for a parrticular frame element

12 June 2024 3,426 1 View

How to get 2 Octanol from Castor oil at a laboratory scale ?

Any latest method developed

24 May 2024 2,128 1 View

What criteria define a tree species as monodominant within a given ecosystem?

I am looking for criteria to define a species as monodominant, based on its DBH and presence locations in the field.

13 May 2024 7,716 3 View

Odd structures in transmission electron microscopy?

Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco

09 May 2024 2,070 11 View

Similar questions and discussions
Which cell line will be the reference for human neuroblastoma?

We are implementing a grant related to the study of supertargets in secondary cultures of human neuroblastoma cells - IMR-32, Kelly, SH-SY5Y, SK-N-AS etc. Comparing gene expression between cell...

16 April 2024 7,706 2 View

Protein extraction from SH-SY5Y neuroblastoma cells and differentiated neurons?

I am working with SH-SY5Y cells and differentiating them into neurons using retinoic acid. I need to extract protein from them in order to conduct a western blot, however, I am having trouble...

31 January 2024 5,056 3 View

Can anyone identify what type of cell culture contamination this is?

Hello, Our lab is struggling with contamination despite taking every precaution necessary to maintain a sterile environment. The images shown are mature iPSC-derived neurons, but the...

03 October 2023 9,110 4 View

Any retinoic acid protocols for differentiating IMR32s?

Hi all, I am trying to differentiate my IMR32 neuroblastoma cells using retinoic acid or a similar treatment, and was wondering if anyone had any protocols you've had success with that you would...

10 August 2023 8,042 0 View

Contamination in sk-n-sh neuroblastoma cell culture?

Hi, I’m trying to culture sk-n-sh neuroblastoma cells. After 8th day of the culture cell morphology disappears and the accumulated structure appears (Please see the photographs). Could anyone make...

29 July 2023 4,952 0 View

¿What abtibodies can I use to evaluate oxidative stress in a cell line Using immunofluorescence ?

Hi, I want to evaluate oxidative stress in a neuroblastoma cell line, I know about SOD1, but I think it's not enough, so I was investigating and I found 4-hydroxynonenal, I have this in my lab...

27 June 2023 9,084 3 View

Which are the best conditions to transfect SK-N-SH with Nucleofector (Amaxa)?

We want to transfect SK-N-SH cells with Nucleofector (Amaxa), but our device don´t include the proper program to do it (X-005 or X-05). Does anybody know which are the settings corresponding to...

15 March 2023 499 3 View

How long would it take to differentiate SHSY5Y cells with retinoic acid?

Hi all, I am trying to differentiate SHSY5Y human neuroblastoma cells into neurons with the retinoic acid protocol. I add 10uM retinoic acid into 3% FBS containing DMEM for three days, and...

21 February 2023 8,474 4 View

Looking for human neuroblastoma cell lines from different stages of disease?

Hello everyone. I am looking for human neuroblastoma cell lines from different stages of disease. To clarify, i am looking for NB cell lines that are representing patients with different stages of...

25 January 2023 5,054 0 View

What is the most common normal neuronal cell lines ?

Dear all! now im working on my thesis (Anti Cancer Effect of Multi walled carbon Nanotubes ). im using SH-SY5Y neuroblastoma cell line and i need a normal neuronal cell line in order to test the...

21 December 2022 4,604 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close