Do you know researchers studying bacterial gene expression you recommend me?

09 October 2021 0 9K Report

I would like to know:

1. If there is any difference between sense and antisense strand in genomic expressión.

2. How is regulated de number of transcribed copies of mRNA, for example, the number of copies of virB2 gene for pili-like construction during the Secretion System Type 4 assembly.

More Adrian Muñoz-Bucio's questions See All

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

Can my lysis buffer interfere with the quantification of DNA and RNA using Qubit2.0?

I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8...

17 January 2021 1,453 3 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

No title

It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

How to correct a deviation in the molar ratio in an Isothermal Titration Calorimetry equipment?

Our equipment of ITC does not reach the molar ratio (N=1) of a titration of EDTA with Ca2+ in MES buffer pH 6. We have cleaned with DCN 90 and HCl pH 2, but the N value is very low, around...

10 November 2020 3,137 3 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

Karl Marx vrs Adam Smith, can you see the market illusion associated with each model?

If there are sustainability gaps, then there are market illusions as well as broken circular economic structures. Hence there is a market illusion associated with red socialism/Karl Marx and with...

15 October 2020 1,538 8 View

If the daily (hourly) concentration of PM2.5 is not Gaussian. Is correct apply an arithmetic mean in epidemiological studies for risk assessment?

The distribution of daily PM2.5 concentration (hourly) is bimodal in the city of Coyhaique (South of Chile). Coyhaique is the city more polluted by particulate matter accord the WHO. The major...

11 October 2020 2,006 3 View

What is a green market? What is not a green market?

Despite green market thinking being here formally since 2012 Rio +20 conference finding a definition of a true green market is hard to find even in major publications dealing with green growth and...

11 October 2020 7,951 24 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to apply predefined temperature to generate prestressed force in Abaqus?

Dear researcher, I'm applying a thermal strain to generate the prestressed force in the steel strand of my concrete wall model using predefined temperature in Abaqus as shown in Figure 1. In...

26 January 2021 7,058 7 View

How to convert ng/ul of DNA concentration to pmol/ul ?

Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...

21 January 2021 2,671 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Is it possible for peeling off pedot:pss film on bare glass?

I try to fabricate free stranding pedot:pss film. I already prepare pedot:pss film by using OTS treated glass. But in many papers, they just using bare glass not OTS treated glass. I did it, but I...

05 January 2021 6,968 1 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

Will these existing vaccine be effective to this new corona variant?

Suggest some good articles on Genomics of this new COVID strain.

03 January 2021 7,635 13 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

Which is the best gene editing tool among TALENs, ZFNs, CRISPR and HENs?

Since two decades we came across many tools to edit the desired gene and genome viz., clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), (2)...

27 December 2020 7,667 10 View