Is there a dataset of atmospheric river tracks for the whole continental US?

I'm looking for the period 1960-2010, so the dataset would most likely have to be based on re-analysis data. Most things I found are for the West Coast only and often just feature the landfalling...

18 January 2021 3,466 2 View

How is single molecule's fluorescence lifetime depending on excitation density?

It is known, that the fluorescence lifetime of an ensemble of molecules being in close vicinity can change, as intermolecular processes between excited chromophores (e.g. superradiance,...

28 October 2020 7,656 3 View

Minimum participants for a focus group interview ?

I am involved in qualitative research and at the time working on the methodological background. My colleague, which was doing the interviews, brought to me they did some focus group interviews....

30 March 2020 4,000 16 View

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10 June 2019 6,662 4 View

How to mesure fluorescence excitation intensity under microscope ?

Hi everyone, My problem is quite simple: I would like to get the fluorescence excitation intensity in mol.m-2.s-1 (or W.m-2). And I didn't find any protocol/advice online to measure it. I...

15 January 2019 9,583 2 View

What insulin receptor antibodies do you recommend for WB and ICC on rat neurons (Santa Cruz sc-710 and sc-711 aren't sold anymore)?

My stock of sc-711 is gone, and SC has been prohibited from selling polyclonal antibodies. I want to do both WB (could be either IRalpha or IRbeta) and ICC, especially unpermeabilized cells (so...

15 January 2018 2,474 3 View

Who knows talented prospective PhD students who want to pursue an academic career in sustainability science?

27 April 2017 2,147 2 View

Which method should we use for analyzing a sample of 45 companies at two points in time?

25 February 2016 1,868 3 View

Can anyone help with co-localization using confocal laser scanning microscopy?

Dear all, I try to identify the cell type (lamina propria) bearing a receptor which I stained (DAB, and AlexaFluor-546) on paraffin-embedded human colonic tissue. Previous co-localizations of...

14 August 2014 447 4 View

Is social value orientation (SVO) biased due to expectations?

05 June 2014 5,446 6 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View